Claudia haarmann nanion patch

We have developed an automated patch clamp module for highthroughput ion channel screening, recording from 384 cells simultaneously. Abstract in recent years, human stem cellderived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. N fertig is the ceo of nanion technologies while all other authors are employees of nanion. The best clones were used to implement light stimulation on an hts automated patch clamp platform synchropatch 384pe, nanion technologies. Launch of first product porta patch in 2003q4 nanion achieves break even in 2004 nanion s patchliner was launched in 2006q1 nanion s syncropatch 96 channel launched in 2011. One of the advantages of planar patch clamp is the possibility of perfusing the intracellular side of the membrane during a patch clamp experiment in. Pdf an update on the advancing highthroughput screening. The module is incorporated into a laboratory pipetting robot and uses a 384channel pipettor. Cecilia farre senior scientist, nanion technologies gmbh, erzgiessereistr.

Patch clamp is the gold standard for obtaining highly relevant information about ion channels and their effectors but it is a notoriously laborious technique. Goetze1, markus rapedius 1, claudia haarmann1, ilka rinke, nadine becker, takayuki oka 2, atsushi ohtsuki, timo stengel 1, marius vogel. Claudia haarmann nanion technologies gmbh, munich, germany. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience tracking cookies. Alison obergrussberger, claudia haarmann, sonja stolzlefeix, nadine becker, atsushi ohtsuki, andrea bruggemann, michael george and niels fertig, automated patch clamp recordings of human stem cellderived cardiomyocytes, stem cellderived models in toxicology, 10. The syncropatch 384pe nanion technologies described here is an automated patch clamp module in combination with a 384channel digital amplifier tecella, a biomek fx pipetting robot beckman coulter with 384 pipettor, software for control of the robot biomek software, beckman coulter, and proprietary software for data acquisition. A useful modification of this approach is the replacement of the glass pipet with a.

Automated patch clamp recordings of human stem cellderived. Pdf high throughput pharmacology of cardiac ltype ca2. Syncropatch 384pe a predecessor model of syncropatch 384i and icon pl. Automated patch clamp meets highthroughput screening. Claudia haarmann nanion technologies, munich researchgate. A useful modification of this approach is the replacement of the glass pipet with a thin planar glass layer with a small hole in the middle. Claudia haarmann director for product implementation apc. Microchip technology for automated and parallel patch. Highthroughput automated patch clamp complemented by. The kinetic time to peak is 1015% faster on the syncropatch 384i using a ligand application of 60 ls instead of 40 ls. The dynamite 8 simulates ion channel currents in cells in realtime during patch. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. The highthroughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Claudia haarmann, director of product implementation apc prod.

The here utilized medium throughput apc device is a planar patch clamp. Our efficient and highly versatile products maximize the output in ion channel screening, safety testing and scientific research. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. Bridging the gap between primary and secondary syncropatch. Doerr, markus rapedius, claudia haarmann, ilka rinke, tom goetze, matthias beckler, michael george, andrea bruggemann and niels fertig nanion technologies, ganghoferstr. The medium throughput patch clamp device used here was a patchliner system nanion technologies, germany. Expert opinion on therapeutic targets volume 11, 2007 issue 4. Reliable identification of herg liability in drug discovery by automated patch clamp introduction conclusions michael george1, rodolfo haedo2, nina brinkwirth1, nadine becker1, claudia haarmann1, alison obergrussberger1, ronald knox2, martin hampl1, niels fertig1 1 nanion technologies, munich, germany, 2nanion technologies inc, livingston, nj, usa email. Sonja stoelzles research works nanion technologies. Specifically, nanion s electrophysiology workstations, the porta patch and the patchliner, have been successfully introduced as highquality automated patch clamp platforms for.

Director of product implementation at nanion technologies. Specifically, nanions electrophysiology workstations, the portapatch and the patchliner, have been successfully introduced as highquality. Sonja stoelzles 28 research works with 2,004 citations and 3,404 reads, including. Frontiers stateoftheart automated patch clamp devices. Nanion technologies is one of the leading providers of high quality automated patch clamp systems. The dynamite 8 simulates ion channel currents in cells in realtime during patch clamp experiments. The syncropatch 384pe nanion technologies described here is an automated patch clamp module in combination with a 384channel digital. Due to their planar chip design not only is a higher throughput achieved, but new applications have also become possible. With this device, nanion offers the worlds smallest patch clamp workstation. Automated patch clamp devices are now commonly used for studying ion channels. An update on the advancing highthroughput screening. Automated patch clamp apc devices have become commonplace in many industrial and academic labs. Claudia haarmann of nanion technologies, munich read 51 publications contact.

Porta patch product flyer internal perfusion porta patch product flyer. Automated patch clamp andrea bruggemann, claudia haarmann, markus rapedius, tom goetze, ilka rinke, michael george, niels fertig. Syncropatch 384i a successor of syncropatch 384pe customer case. Our efficient and highly versatile products maximize the. Nanion is pleased to announce the launch of dynamite 8, a dynamic clamp unit for the multichannel automated patch clamp system, the patchliner. Automated patch clamp and an impedancebased assay system with cardiomyocytes derived from pluripotent stem.

Syncropatch 384pe a predecessor model of syncropatch 384i and icon ce. Mohamed kreir, michael george, sonja stoelzle, claudia haarmann, cecilia farre and alison haythornthwaite. Full dose response curves can be acquired from individual cells reducing the cost per data point. Join facebook to connect with claudia haarmann and others you may know.

To meet the ever increasing demand for higher throughput in ion channel screening and safety testing we have developed a highly parallel patch clamp platform, the syncropatch 96. Cecilia farre, alison haythornthwaite, claudia haarmann, sonja stoelzle. Our efficient and highly versatile products maximize the output in. Faster dispense speed p2x3, 10 m meatp applied with 60 ls, 40 ls and 20 ls.